Remark : best display with netscape communicator !
Using program TACG from bionavigator, the result is following;
Enzymes that DO NOT cut; EcoRI
Enzymes that cut; Sau3AI, HinfI and MseI.
List of cut sites
| Enzyme | Site | # Cuts | Sites |
| HinfI | G'AnT_C | 1 | 151 |
| MseI | T'TA_A | 1 | 167 |
| Sau3AI | 'GATC_ | 3 | 39 161 214 |
Linear Map of Sequence:
AlwI AciI
CviRI Sau3AI MspA1I Pfl1108I
BccI TaiI BsmI DpnI FauI
\ \ \ \ \ \ \ \ \ \ \
1 aaaaccatcaaatacgttatgctggaatgcaacgaactgatcccgctgttctacgaaacc 60
ttttggtagtttatgcaatacgaccttacgttgcttgactagggcgacaagatgctttgg
^ * ^ * ^ * ^ * ^ * ^ *
1 K T I K Y V M L E C N E L I P L F Y E T
CviJI
MspI
ScrFI
NciI Pfl1108I
BspMI CjePI HphI NlaIII CjePI
\ \ \ \ \ \ \
61 tgcccggctggtgaaaacatctgctacgaaagttcatggttgctaccccgaaagttccgt 120
acgggccgaccacttttgtagacgatgctttcaagtaccaacgatggggctttcaaggca
^ * ^ * ^ * ^ * ^ * ^ *
1 C P A G E N I C Y E S S W L L P R K F R
MboII TfiI
MspI
ScrFI MseI TseI
TaqI TaiI NciI Sau3AI Fnu4HI
TaiI CviRI SfaNI HinfI DpnI BbvI TaiI
\ \ \ \ \ \ \ \ \ \ \ \ \\
121 gcgaacgtggttgcatcgacgtttgcccggaatcttctctgatcgttaaatacgtttgct 180
cgcttgcaccaacgtagctgcaaacgggccttagaagagactagcaatttatgcaaacga
^ * ^ * ^ * ^ * ^ * ^ *
1 A N V V A S T F A R N L L * S L N T F A
Sau3AI AciI
BsrI HhaI DpnI TauI
BsbI Bst4CI HaeII Fnu4HI
CviRI BsiEI AlwNI TaqI CviJI
\ \ \\ \ \\ \ \ \ \\
181 gcaacaccgaccgttgccagtaatccagcgcctgatctctcgaaataaaagccgcattg 240
cgttgtggctggcaacggtcattaggtcgcggactagagagctttattttcggcgtaac
^ * ^ * ^ * ^ * ^ * ^ *
1 A T P T V A S N P A P D L S K * K P H