Remark : best display with netscape communicator !

Using program TACG from bionavigator, the result is following;

Enzymes that DO NOT cut; EcoRI

Enzymes that cut; Sau3AI, HinfI and MseI.

List of cut sites

 

 

Enzyme Site # Cuts Sites
HinfI G'AnT_C 1 151
MseI T'TA_A 1 167
Sau3AI 'GATC_ 3 39 161 214

 

 

Linear Map of Sequence:

                                           AlwI     AciI                        
                                      CviRI    Sau3AI MspA1I  Pfl1108I          
               BccI     TaiI        BsmI        DpnI        FauI                
               \          \           \ \  \   \ \  \ \    \  \                 
      1   aaaaccatcaaatacgttatgctggaatgcaacgaactgatcccgctgttctacgaaacc     60   
          ttttggtagtttatgcaatacgaccttacgttgcttgactagggcgacaagatgctttgg          
              ^    *    ^    *    ^    *    ^    *    ^    *    ^    *          
1         K  T  I  K  Y  V  M  L  E  C  N  E  L  I  P  L  F  Y  E  T            
                CviJI                                                           
             MspI                                                               
             ScrFI                                                              
             NciI                  Pfl1108I                                     
                BspMI CjePI    HphI            NlaIII        CjePI              
             \  \     \        \   \           \             \                  
     61   tgcccggctggtgaaaacatctgctacgaaagttcatggttgctaccccgaaagttccgt    120   
          acgggccgaccacttttgtagacgatgctttcaagtaccaacgatggggctttcaaggca          
              ^    *    ^    *    ^    *    ^    *    ^    *    ^    *          
1         C  P  A  G  E  N  I  C  Y  E  S  S  W  L  L  P  R  K  F  R            
                                 MboII TfiI                                     
                                    MspI                                        
                                    ScrFI              MseI        TseI         
                         TaqI  TaiI NciI         Sau3AI             Fnu4HI      
                 TaiI CviRI    SfaNI   HinfI       DpnI   BbvI  TaiI            
                 \    \  \     \ \  \  \         \ \   \  \     \  \\           
    121   gcgaacgtggttgcatcgacgtttgcccggaatcttctctgatcgttaaatacgtttgct    180   
          cgcttgcaccaacgtagctgcaaacgggccttagaagagactagcaatttatgcaaacga          
              ^    *    ^    *    ^    *    ^    *    ^    *    ^    *          
1         A  N  V  V  A  S  T  F  A  R  N  L  L  *  S  L  N  T  F  A            
                                          Sau3AI             AciI               
                        BsrI           HhaI DpnI             TauI               
             BsbI    Bst4CI             HaeII                Fnu4HI             
          CviRI     BsiEI               AlwNI    TaqI       CviJI               
          \  \      \\  \              \\ \ \    \          \\                  
    181   gcaacaccgaccgttgccagtaatccagcgcctgatctctcgaaataaaagccgcattg     240   
          cgttgtggctggcaacggtcattaggtcgcggactagagagctttattttcggcgtaac           
              ^    *    ^    *    ^    *    ^    *    ^    *    ^    *          
1         A  T  P  T  V  A  S  N  P  A  P  D  L  S  K  *  K  P  H